3D-12 Positional Cloning of the Genes Determining Heterostylous Self-incompatibility in Buckwheat

Since the age of C. Darwin, the different forms of flowers have been attracted many biologists. Fascinatingly, in a few plant species, multiple forms of flowers are tightly associated with self-incompatibility, exhibiting so called heteromorphic self-incompatibility, whose molecular mechanism is so...

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Published inGenes & Genetic Systems Vol. 81; no. 6; p. 444
Main Authors OTA Tatsuya, MORI Masashi, MORI Mari, MATSUMOTO Daiki, OHNISHI Ohmi, CAMPBELL Clayton, YASUI Yasuo
Format Journal Article
LanguageJapanese
Published The Genetics Society of Japan 2006
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Summary:Since the age of C. Darwin, the different forms of flowers have been attracted many biologists. Fascinatingly, in a few plant species, multiple forms of flowers are tightly associated with self-incompatibility, exhibiting so called heteromorphic self-incompatibility, whose molecular mechanism is so far little known. In order to elucidate the molecular mechanism and evolutionary course of heteromorphic self-incompatibility, we have initiated positional cloning of the genes involved in the heteromorphic self-incompatibility in buckwheat and so far obtained the following：(1)A BAC library of about 7-8 coverage with average insert size of 76kb has been constructed. (2)An AFLP marker, approximately 0.02cM away from S supergene complex, was identified by searching alleles differently expressed between two forms of flowers of inbreeding lines. (3)Result of chromosome walking between two AFLP markers has indicated that recombination rate is suppressed with respect to physical distance at the region near S supergene complex. In the presentation, we are going to update our results on chromosome walking and nucleotide sequencing of BACs currently being conducted.
ISSN:1341-7568