THE MEDAKA FISH TRANSPOSABLE ELEMENT Tol2

Our goal is to establish, in vertebrates, an efficient gene tagging system using Tol2, which is a terminal-inverted-repeat transposable element we found in the medaka fish genome. The purpose of the present study is to detect de nova insertion of Tol2. We injected fertilized eggs with plasmids A and...

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Bibliographic Details
Published inGenes & Genetic Systems Vol. 75; no. 6; p. 382
Main Authors KOGA Akihiko, HORI Hiroshi
Format Journal Article
LanguageJapanese
Published The Genetics Society of Japan 2000
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Summary:Our goal is to establish, in vertebrates, an efficient gene tagging system using Tol2, which is a terminal-inverted-repeat transposable element we found in the medaka fish genome. The purpose of the present study is to detect de nova insertion of Tol2. We injected fertilized eggs with plasmids A and B and recovered plasmid molecules from embryos. Screening with Kan and Tet resulted in detection of plasmid C. The Tol2 portion of plasmid C was flanked by an 8 bp duplication that originates from plasmid B. Transposition was observed when mRNA for a putative transposase was injected with plasmids A and B and not observed without the mRNA.
ISSN:1341-7568
1880-5779