CLONING OF MOUSE TRANSCRIPTION-REPAIR COUPLING FACTOR TrcF GENE

• Published in: Genes & Genetic Systems Vol. 71; no. 6; p. 450
• Main Authors: MORIMYO Mitsuoki, SAITO Toshiyuki, MITA Kazuei, HIGASHI Tomoyasu, AJIMURA Masahiro, SUGAYA Kimihiko, HONGO Etsuko
• Format: Journal Article
• Language: Japanese
• Published: The Genetics Society of Japan 1996
• ISSN: 1341-7568; 1880-5779

Fission yeast S. pombe is assumed to be a good model organism of eucaryotes, because it is one of the simplest eucaryotes whose housekeeping genes are considered to be conserved through eucaryotic evolution. We can, therefore, expect that it supplies a good system for cloning of human DNA repair genes which are one of the most important housekeeping genes. In a study of the number and functions of the housekeeping genes of eukaryotic cells, we chose S. pombe as a model organism and made a catalog of S. pombe cDNA. Among 2700 independent cDNA clones obtained, 900 clones were similar to a gene of other species. We could get 20 DNA repair related genes, 10 new and 10 known genes. Among 10 new DNA repair genes of S. pombe, five human homologs were already obtained but not the rest 5 genes. We have tried to clone a mouse DNA repair gene by the PCR method using the DNA primer which was deduced from the conserved amino acid sequence between a DNA helicase gene of S. pombe and that of S. cerevisiae. We could have three DNA helicase genes which were homologous to RADS gene of S. cerevisiae, mfd gene of E. coli and a transcription elongation factor S-II gene of human. This method could be applied to any genes as far as their protein homology is highly conserved.