Immunopharmacological studies of eugenol

Medicaments combined with eugenol are used for root canal treatment in dentistry. We proved eugenol induces contact dermatitis in mice. In this study the distribution of immunocompetent cells in mice was examined. Male BALB/cAnN SPF mice were sensitized with 20μl of the sample two times at one-day i...

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Bibliographic Details
Published inJapanese Journal of Pharmacology Vol. 64; no. suppl.2; p. 309
Main Authors Tomoko Ohsumi, Sen Higashi, Keiko Ozumi, Kayoko Kuroki
Format Journal Article
LanguageJapanese
Published The Japanese Pharmacological Society 1994
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Summary:Medicaments combined with eugenol are used for root canal treatment in dentistry. We proved eugenol induces contact dermatitis in mice. In this study the distribution of immunocompetent cells in mice was examined. Male BALB/cAnN SPF mice were sensitized with 20μl of the sample two times at one-day intervals. After five days the mice were challenged with 10μl of the sample. The inguinal lymph nodes and ears were sampled at 30 minutes after the sensitization or one day after the challenge. Cryostat sections were prepared and distribution of immunocompetent cells was demonstrated immunohistochemically with the indirect enzyme immunoassay. Three monoclonal antibodies were used as primary antibodies ; anti-Ia (Ia), anti-Thy-1.2 (Thy) and anti-interleukin-2 (IL-2). There was a difference between the distribution of three immunocompetent cells la-positive cells, Thy-positive cells and IL-2-positive cells. The results suggest that the immune response in the lymph node may be brought about by eugenol in the early phase of contact dermatitis.
ISSN:0021-5198
1347-3506