9. Effects of Azithromycin on Osteoclast Differentiation

• Published in: Dental Medicine Research Vol. 32; no. 1; p. 44
• Main Authors: Md.Zahirul Hague Bhuyan, Kakei Ryu, Shoji Yamada, Hitoshi Amano
• Format: Journal Article
• Language: Japanese
• Published: Showa University Dental Society 31.03.2012
• ISSN: 1882-0719

Azithromycin (AZM), a member of the macrolide group of drugs, is an antibiotic, and function as an anti-inflammatory drug. 「Materials and Methods」Bone marrow cells from femur and tibia of ddY four/five-week-old mice passed Sephadex G10. The cells were cultured with pH 7.0 αMEM, which include CSF-1 (25 ng/ml) and RANKL (10 ng/ml), with or without AZM (0-10 μg/ml). RAW 264.7 cells for this experiment are the same as before, using only RANKL. An in vivo experiment was performed using ddY 5-week-old mice. The experimental group was injected with AZM (10 mg/kg), and after 30 min 0.9% acetic acid was injected intraperitoneally. The control group was only injected with acetic acid and the stretching number of both group. On day 4 and day 7, both groups were injected with only acetic acid. 「Results」After 6 days, both cells were differentiated to TRAP positive multinucleated osteoclasts. The number of osteoclast decreased as the AZM dose increased. In the in vivo experiment, the stretch number of the experimental group was higher on day 7 then it was on day 0. 「Discussion」Differentiation of hematopoietic stem cells and RAW 264.7 cells into mature osteoclasts decreased as a result of AZM treatment. Injection of dilute acetic acid solution intraperitoneally caused a writhing response in the control group. This reaction was significantly reduced by pretreatment with AZM. 「Conclusion」These results provide evidence for the important finding that AZM effects osteoclast differentiation and possesses anti-inflammatory action.