Study on Cerebral Microvasculature and Cerebral Vascular Bed Volume in Acute Brain Swelling using Immunoflorescence Method and Quantitative Immunoelectrophoresis

• Published in: Neurologia medico-chirurgica Vol. 13; no. 1; p. 146
• Main Authors: Takeshi KIMOTO, Kenju WATANABE, Kohki NOSAKA, Takeshi SHIMA, Susumu ISHIKAWA, Akira IKEDA
• Format: Journal Article
• Language: Japanese
• Published: THE JAPAN NEUROSURGICAL SOCIETY 1973
• ISSN: 0470-8105

In the occurrence of acute brain swelling, the increase in the volume of the cerebral vascular bed has attracted the attention as a contributing factor. The present study was attempted to detect the alterations in the volume of the cerebral vascular bed by observing the morphological changes in microvasculature with immunofluorescence method and by evaluating the amount of intravascular serum albumin in acutely swollen brain of the dog. Acute brain swelling was produced with epidural balloon method. The swollen brain was frozen instantaneously with liquid nitrogen and fixed in cold alcohol containing acetic acid. Slices of 50-80μ in thickness were made and incubated with FITC-labelled antibody against dog serum albumin. Microvasculature pattern was observed under fluorescein microscope. The same study was carried out also in the normal brain. For evaluating the amount of the intravascular serum albumin, the frozen brain was sectioned and cut into small blocks of 250μ×1.25mm ×1.25mm in size. Soluble protein containing serum albumin was eluted from these blocks quantitatively. Amount of serum albumin was determined by quantitative immunoelectrophoresis (modification of Laurells method) of the soluble protein with antibody against dog serum albumin. The same procedures were performed also in the normal brain.