Identification of Autoantigens in Pediatric Gastric Juices

Purpose: This study aimed to investigate the presence of autoantigens in the gastric juices of children. Methods: Gastric juice and serum samples were obtained from 53 children <15 years of age who underwent gastric endoscopy. Among these, 8, 22, and 23 participants were in the age groups 0-5, 6-...

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Published inPediatric gastroenterology, hepatology & nutrition Vol. 27; no. 1; pp. 15 - 25
Main Authors Hee-Shang Youn, Jin-Su Jun, Jung Sook Yeom, Ji Sook Park, Jae-Young Lim, Hyang-Ok Woo, Jung-Wook Yang, Seung-Chul Baik, Woo-Kon Lee, Ji-Hyun Seo
Format Journal Article
LanguageKorean
Published 2024
Subjects
Helicobacter pylori
Autoantigens
Children
Gastric juice
Gastritis
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Summary:Purpose: This study aimed to investigate the presence of autoantigens in the gastric juices of children. Methods: Gastric juice and serum samples were obtained from 53 children <15 years of age who underwent gastric endoscopy. Among these, 8, 22, and 23 participants were in the age groups 0-5, 6-10, and 11-15 years, respectively. These samples were analyzed using two-dimensional electrophoresis (2-DE), immunoblot analysis, and matrix-assisted laser desorption ionization-time of-flight mass spectrometry. Furthermore, we reviewed the histopathological findings and urease test results and compared them with the results of 2-DE and immunoblot analysis. Results: There were no statistically significant differences in urease test positivity, grades of chronic gastritis, active gastritis, or Helicobacter pylori infiltration of the antrum and body among the three age groups. Three distinct patterns of gastric juice were observed on 2-DE. Pattern I was the most common, and pattern III was not observed below the age of 5 years. Histopathological findings were significantly different among active gastritis (p=0.037) and H. pylori infiltration (p=0.060) in the gastric body. The immunoblots showed large spots at an approximate pH of 3-4 and molecular weights of 31-45 kDa. These distinct, large positive spots were identified as gastric lipase and pepsin A and C. Conclusion: Three enzymes, which are normally secreted under acidic conditions were identified as autoantigens. Further investigation of the pathophysiology and function of autoantigens in the stomach is required.
Bibliography:KISTI1.1003/JNL.JAKO202408043270820
ISSN:2234-8646
2234-8840