Single-Cell Molecular Barcoding to Decode Multimodal Information Defining Cell States

Single-cell research has provided a breakthrough in biology to understand heterogeneous cell groups, such as tissues and organs, in development and disease. Molecular barcoding and subsequent sequencing technology insert a single-cell barcode into isolated single cells, allowing separation cell by c...

Full description

Saved in:
Bibliographic Details
Published inMolecules and cells Vol. 46; no. 2; pp. 74 - 85
Main Author Ik Soo Kim
Format Journal Article
LanguageKorean
Published 2023
Subjects
molecular barcoding
single cell
multimodality
multiomics
Online AccessGet full text

Cover

More Information
Summary:Single-cell research has provided a breakthrough in biology to understand heterogeneous cell groups, such as tissues and organs, in development and disease. Molecular barcoding and subsequent sequencing technology insert a single-cell barcode into isolated single cells, allowing separation cell by cell. Given that multimodal information from a cell defines precise cellular states, recent technical advances in methods focus on simultaneously extracting multimodal data recorded in different biological materials (DNA, RNA, protein, etc.). This review summarizes recently developed single-cell multiomics approaches regarding genome, epigenome, and protein profiles with the transcriptome. In particular, we focus on how to anchor or tag molecules from a cell, improve throughputs with sample multiplexing, and record lineages, and we further discuss the future developments of the technology.
Bibliography:KISTI1.1003/JNL.JAKO202316041148740
ISSN:1016-8478
0219-1032