The efficiency of different types of extenders for semen cooling in stallions

• Published in: Animal bioscience Vol. 35; no. 5; pp. 670 - 676
• Main Authors: Reckova, Zuzana, Filipcík, Radek, Souskova, Katarína, Kopec, Tomas, Hosek, Martin, Pesan, Vojtech
• Format: Journal Article
• Language: Korean
• Published: 2022
• Subjects: Semen Extender; Stallion; Fertilizing Capacity; Semen Cooling
• ISSN: 2765-0189; 2765-0235

Objective: This study was conducted to examine influence of skimmed milk-based extender (SM), INRA 96 extender and BotuSemen Gold extender on parameters of stallions' ejaculate during storage. Methods: In this study, 14 stallions between 4 and 20 years of age were monitored. Total and progressive motility, viability and morphology of sperm were evaluated at time intervals of 24, 48, and 72 hours after collection. Results: The total motility, progressive motility, and values of sperm with normal morphology were significantly higher in the INRA 96 and BotuSemen Gold extenders than in the SM (p<0.01). The sperm viability differed significantly in all extenders (p<0.01). The highest value of sperm viability was in INRA 96 (64.69%±0.67%) and lowest in SM (59.70%±0.81%). The highest differences occurred at 72 hours of storage. Values of total motility, progressive motility and sperm viability decreased over time (p<0.01). In case of sperm morphology there was no statistically significant decrease between 48- and 72-hour time intervals. Conclusion: It can be concluded that the extenders with a chemically defined composition have shown better indicators of insemination capabilities in ejaculates than the SM. The BotuSemen Gold extender is a suitable alternative to the INRA 96, when used within 48 hours; after 72 hours of storage, however, the INRA 96 showed a higher share of viable spermatozoa.