Serological evidence of West Nile viral infection in archived swine serum samples from Peninsular Malaysia

West Nile virus (WNV), a neurotropic arbovirus, has been detected in mosquitos, birds, wildlife, horses, and humans in Malaysia, but limited information is available on WNV infection in Malaysian pigs. We tested 80 archived swine serum samples for the presence of WNV antibody and West Nile (WN) vira...

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Published inJournal of veterinary science (Suwŏn-si, Korea) Vol. 22; no. 3; pp. 29.1 - 29.6
Main Authors Mohammed, Mohammed Nma, Yasmin, Abd Rahaman, Noraniza, Mohd Adzahan, Ramanoon, Siti Zubaidah, Arshad, Siti Suri, Bande, Faruku, Mohammed, Hussni O
Format Journal Article
LanguageKorean
Published 2021
Subjects
pigs
West Nile virus
RT-PCR
Malaysia
ELISA
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Summary:West Nile virus (WNV), a neurotropic arbovirus, has been detected in mosquitos, birds, wildlife, horses, and humans in Malaysia, but limited information is available on WNV infection in Malaysian pigs. We tested 80 archived swine serum samples for the presence of WNV antibody and West Nile (WN) viral RNA using ID Screen West Nile Competition Multi-species enzyme-linked immunosorbent assay kits and WNV-specific primers in reverse transcription polymerase chain reaction assays, respectively. A WNV seroprevalence of 62.5% (50/80) at 95% confidence interval (51.6%-72.3%) was recorded, with a significantly higher seroprevalence among young pigs (weaner and grower) and pigs from south Malaysia. One sample was positive for Japanese encephalitis virus antibodies; WN viral RNA was not detected in any of the serum samples.
Bibliography:KISTI1.1003/JNL.JAKO202111852373840
ISSN:1229-845X
1976-555X