Plant Regeneration of Iris koreana Nakai through Organogenesis for Ex-situ Conservation

Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also inv...

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Bibliographic Details
Published inJournal of forest and environmental science Vol. 37; no. 4; pp. 304 - 308
Main Authors Bae, Kee-Hwa, Yun, I-Seul, Jung, Ji-Sun, Kim, Chan-Beom, Kim, Hye-Won, Hong, Yong-Sik, Oak, Min-Kyeong, Kim, Hak-Koo, Lee, Ju-Hui
Format Journal Article
LanguageKorean
Published 2021
Subjects
proliferation
callus
in vitro
Iris koreana
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Summary:Iris koreana (Iridaceae) is an endangered plant native to Korea. In order to develop an in vitro propagation method, we investigated the effect of 2,4-dichlorophenoxy acetic acid (2,4-D) and a-naphthalene acetic acid (NAA) on callus induction in different I. koreana tissues. In addition, we also investigated the effect of 2,4-D and Benzyl aminopurine (BA) treatments on adventitious shoot induction in viable calli and the effect of indole-3-butyric acid (IBA) on root formation in viable shoots. We found that callus production was highest with 1.0 mg/L NAA (94.4% cultured rhizome explants), and adding low concentrations of 2,4-D to BA containing media significantly increased the frequency of shoot primordial formation. The best rooting results were obtained with 1.0 mg/L IBA, on which 98% of regenerated shoots developed roots and produced an average of 7.4 roots within 45 days. This in vitro propagation protocol will be useful for conservation, as well as for mass propagation.
Bibliography:KISTI1.1003/JNL.JAKO202107554229237
ISSN:2288-9744
2288-9752