Ginsenoside Rg1 from Panax ginseng enhances myoblast differentiation and myotube growth

Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibrobl...

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Bibliographic Details
Published inJournal of ginseng research Vol. 41; no. 4; pp. 608 - 614
Main Authors Go, Ga-Yeon, Lee, Sang-Jin, Jo, Ayoung, Lee, Jaecheol, Seo, Dong-Wan, Kang, Jong-Sun, Kim, Si-Kwan, Kim, Su-Nam, Kim, Yong Kee, Bae, Gyu-Un
Format Journal Article
LanguageKorean
Published 2017
Subjects
promyogenic signaling
myoblast differentiation
ginsenoside Rg1
myogenic conversion
myotube hypertrophy
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Summary:Background: Ginsenoside Rg1 belongs to protopanaxatriol-type ginsenosides and has diverse pharmacological activities. In this report, we investigated whether Rg1 could upregulate muscular stem cell differentiation and muscle growth. Methods: C2C12 myoblasts, MyoD-transfected 10T1/2 embryonic fibroblasts, and HEK293T cells were treated with Rg1 and differentiated for 2 d, subjected to immunoblotting, immunocytochemistry, or immunoprecipitation. Results: Rg1 activated promyogenic kinases, p38MAPK (mitogen-activated protein kinase) and Akt signaling, that in turn promote the heterodimerization with MyoD and E proteins, resulting in enhancing myogenic differentiation. Through the activation of Akt/mammalian target of rapamycin pathway, Rg1 induced myotube growth and prevented dexamethasone-induced myotube atrophy. Furthermore, Rg1 increased MyoD-dependent myogenic conversion of fibroblast. Conclusion: Rg1 upregulates promyogenic kinases, especially Akt, resulting in improvement of myoblast differentiation and myotube growth.
Bibliography:KISTI1.1003/JNL.JAKO201731242007756
ISSN:1226-8453
2093-4947