The effect of the site of laser zona opening on the complete hatching of mouse blastocysts and their cell numbers

• Published in: Clinical and experimental reproductive medicine Vol. 43; no. 3; pp. 152 - 156
• Main Authors: Sanmee, Usanee, Piromlertamorn, Waraporn, Vutyavanich, Teraporn
• Format: Journal Article
• Language: Korean
• Published: 2016
• Subjects: Hatching; Lasers; Blastocyst; Zona pellucida; Inner cell mass
• ISSN: 2233-8233; 2233-8241

Objective: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. Methods: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125). Results: The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84.8% vs 80.8%, respectively; p=0.402), but was significantly lower in the control group (51.2%, p<0.001). The cell numbers in the completely hatched blastocysts were comparable in the control group, the ICM group, and the TE group ($69{\pm}19.3$, $74{\pm}15.7$, and $71{\pm}16.8$, respectively; p=0.680). Conclusion: These findings indicate that the site of laser zona opening did not influence the rate of complete hatching of mouse blastocysts or their cell numbers.