Antifilarial and Antibiotic Activities of Methanolic Extracts of Melaleuca cajuputi Flowers

We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays....

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Published inKorean journal of parasitology Vol. 54; no. 3; pp. 273 - 280
Main Authors Al-Abd, Nazeh M, Nor, Zurainee Mohamed, Mansor, Marzida, Hasan, MS, Kassim, Mustafa
Format Journal Article
LanguageKorean
Published 2016
Subjects
lymphatic filariasis
Brugia pahangi
Wolbachia
Melaleuca cajuputi
natural product
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Summary:We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.
Bibliography:KISTI1.1003/JNL.JAKO201624240304056
ISSN:0023-4001
1738-0006