Magnesium Uptake by the Green Microalga Chlorella vulgaris in Batch Cultures

• Published in: Journal of microbiology and biotechnology Vol. 26; no. 3; pp. 503 - 510
• Main Authors: Ayed, Hela Ben Amor-Ben, Taidi, Behnam, Ayadi, Habib, Pareau, Dominique, Stambouli, Moncef
• Format: Journal Article
• Language: Korean
• Published: 2016
• Subjects: Chlorella vulgaris; absorbed Mg2+ (intracellular); metal ions uptake; photobioreactor; adsorbed Mg2+ (extracellular)
• ISSN: 1017-7825; 1738-8872

The accumulation (internal and superficial distribution) of magnesium ions (Mg2+) by the green freshwater microalga Chlorella vulgaris (C. vulgaris) was investigated under autotrophic culture in a stirred photobioreactor. The concentrations of the three forms of Mg2+ (dissolved, extracellular, and intracellular) were determined with atomic absorption spectroscopy during the course of C. vulgaris growth. The proportions of adsorbed (extracellular) and absorbed (intracellular) Mg2+ were quantified. The concentration of the most important pigment in algal cells, chlorophyll a, increased over time in proportion to the increase in the biomass concentration, indicating a constant chlorophyll/biomass ratio during the linear growth phase. The mean-average rate of Mg2+ uptake by C. vulgaris grown in a culture medium starting with 16 mg/l of Mg2+ concentration was measured. A clear relationship between the biomass concentration and the proportion of the Mg2+ removal from the medium was observed. Of the total Mg2+ present in the culture medium, 18% was adsorbed on the cell wall and 51% was absorbed by the biomass by the end of the experiment (765 h). Overall, 69% of the initial Mg2+ were found to be removed from the medium. This study supported the kinetic model based on a reversible first-order reaction for Mg2+ bioaccumulation in C. vulgaris, which was consistent with the experimental data.