Ginsenoside Re Inhibits Osteoclast Differentiation in Mouse Bone Marrow-Derived Macrophages and Zebrafish Scale Model

• Published in: Molecules and cells Vol. 39; no. 12; pp. 855 - 861
• Main Authors: Park, Chan-Mi, Kim, Hye-Min, Kim, Dong Hyun, Han, Ho-Jin, Noh, Haneul, Jang, Jae-Hyuk, Park, Soo-Hyun, Chae, Han-Jung, Chae, Soo-Wan, Ryu, Eun Kyoung, Lee, Sangku, Liu, Kangdong, Liu, Haidan, Ahn, Jong-Seog, Kim, Young Ock, Kim, Bo-Yeon, Soung, Nak-Kyun
• Format: Journal Article
• Language: Korean
• Published: 2016
• Subjects: RANKL; osteoclasts; ginsenoside Re; zebrafish
• ISSN: 1016-8478; 0219-1032

Ginsenosides, which are the active materials of ginseng, have biological functions that include anti-osteoporotic effects. Aqueous ginseng extract inhibits osteoclast differentiation induced by receptor activator of NF-${\kappa}B$ ligand (RANKL). Aqueous ginseng extract produces chromatography peaks characteristic of ginsenosides. Among these peaks, ginsenoside Re is a major component. However, the preventive effects of ginsenoside Re against osteoclast differentiation are not known. We studied the effect of ginsenoside Re on osteoclast differentiation, RANKL-induced tartrate-resistant acid phosphatase (TRAP) activity, and formation of multinucleated osteoclasts in vitro. Ginsenoside Re hampered osteoclast differentiation in a dose-dependent manner. In an in vivo zebrafish model, aqueous ginseng extract and ginsenoside Re had anti-osteoclastogenesis effects. These findings suggest that both aqueous ginseng extract and ginsenoside Re prevent bone resorption by inhibiting osteoclast differentiation. Ginsenoside Re could be important for promoting bone health.