Imaging Single-mRNA Localization and Translation in Live Neurons

Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translati...

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Bibliographic Details
Published inMolecules and cells Vol. 39; no. 12; pp. 841 - 846
Main Authors Lee, Byung Hun, Bae, Seong-Woo, Shim, Jaeyoun Jay, Park, Sung Young, Park, Hye Yoon
Format Journal Article
LanguageKorean
Published 2016
Subjects
live-cell imaging
single-molecule imaging
MS2-GFP
RNA localization
local translation
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Summary:Local protein synthesis mediates precise spatio-temporal regulation of gene expression for neuronal functions such as long-term plasticity, axon guidance and regeneration. To reveal the underlying mechanisms of local translation, it is crucial to understand mRNA transport, localization and translation in live neurons. Among various techniques for mRNA analysis, fluorescence microscopy has been widely used as the most direct method to study localization of mRNA. Live-cell imaging of single RNA molecules is particularly advantageous to dissect the highly heterogeneous and dynamic nature of messenger ribonucleoprotein (mRNP) complexes in neurons. Here, we review recent advances in the study of mRNA localization and translation in live neurons using novel techniques for single-RNA imaging.
Bibliography:KISTI1.1003/JNL.JAKO201606776009969
ISSN:1016-8478
0219-1032