Prognostic Significance of Hes-1, a Downstream Target of Notch Signaling in Hepatocellular Carcinoma

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 16; no. 9; pp. 3811 - 3816
• Main Authors: Zou, Jing-Huai, Xue, Tong-Chun, Sun, Chun, Li, Yan, Liu, Bin-Bin, Sun, Rui-Xia, Chen, Jie, Ren, Zheng-Gang, Ye, Sheng-Long
• Format: Journal Article
• Language: Korean
• Published: 2015
• Subjects: lung metastasis; Shh; notch-1; Hepatocellular carcinoma; Hes-1
• ISSN: 1513-7368; 2476-762X

Background: Hairy and enhancer of split 1 (Hes-1) protein is a downstream target of Notch signaling and is a basic helix-loop-helix transcriptional repressor. However, definitive evidence for a role in hepatocellular carcinoma (HCC) cells has not been reported. Here, Hes-1 was revealed to an important component of the Notch signaling cascade in HCC cell lines possessing different potential for lung metastasis. Materials and Methods: RNAi mediated by plasmid constructs was used to analyze the role of Hes-1 in MHCC-97L HCC cells by assessing proliferation, apoptosis, cell migration and matrigel invasion following transfection. Hes-1 protein expression analysis in HCC tissue was also conducted by immunohistochemistry. Results: Our studies revealed that Hes-1 was decreased in HCC cell lines with higher lung metastasis potential at both the mRNA and protein levels. Down-regulation of the Hes-1 gene in MHCC-97L cells resulted in increased cell proliferation, reduced apoptosis and increased migration and invasion. Conclusions: Hes-1 has potential prognostic value in post-surgical HCC patients and may be an independent prognostic indicator for overall survival and tumor recurrence. These findings have important implications for understanding the mechanisms by which Hes-1 participates in tumor proliferation and invasion.