Synthesis of Hapten for Indirect Competitive Immunoassay for Measuring 3,5,6-trichloro-2-pyridinol

• Published in: Hanguk hwangyeong nonghak hoeji Vol. 34; no. 4; pp. 345 - 349
• Main Authors: Kim, Areumnuri, Kim, Joong-Young, Jeong, Sang-Hee, Cho, Myung-Haing, Park, Kyung-Hun, Cho, Namjun, Paik, Min Kyoung
• Format: Journal Article
• Language: Korean
• Published: 2015
• Subjects: Chlorpyrifos; TCP; Hapten
• ISSN: 1225-3537; 2233-4173

BACKGROUND: In this study, we have attempted to identify a urinary biomarker to assess chlorpyrifos exposure in farmers. The major metabolite and the excretion pathway of chlorpyrifos is 3,5,6-trichloro-2-pyridinol (TCP) in urine. Herein, we describe an adequate synthetic method for TCP hapten for measuring urinary TCP of farmers. METHODS AND RESULTS: First, TCP was prepared by spacer attachment through hydrolysis of thiophosphate ester from chlorpyrifos. After reaction with benzyl bromide, the TCP was transformed into 2,3,5-trichloro-6-benzyloxypyridine. Next, the chlorine in the 2 nd position of the pyridyl ring was substituted into 3-mercaptopropanoic acid spacer arm. Finally, the phenyl group attached to the 6 th position in pyridyl ring was removed for producing the targeted product, 3-(3,5-Dichloro-6-hydroxy-2-pyridyl) thiopropanoic acid. CONCLUSION: Henceforth, this TCP hapten would be used in developing immunoassay studies for the detection and quantitation of urinary TCP of farmers.