Antimicrobial, Antioxidant and Hemolytic Activity of Water-soluble Extract of Mottled Anemone Urticina crassicornis

We evaluated the biological activities of five water extracts of tissue of the mottled anemone Urticina crassicornis. Most extracts exhibited broad-spectrum antimicrobial activity as determined by ultrasensitive radial diffusion assay (URDA) against gram-positive and -negative bacteria, including a...

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Bibliographic Details
Published inFisheries and aquatic sciences Vol. 18; no. 4; pp. 341 - 347
Main Authors Lee, Ye Jin, Kim, Chan-Hee, Oh, Hye Young, Go, Hye-Jin, Park, Nam Gyu
Format Journal Article
LanguageKorean
Published 2015
Subjects
Urticina crassicornis
Hemolysis
Antimicrobial activity
Antioxidant activity
Water-soluble extract
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Summary:We evaluated the biological activities of five water extracts of tissue of the mottled anemone Urticina crassicornis. Most extracts exhibited broad-spectrum antimicrobial activity as determined by ultrasensitive radial diffusion assay (URDA) against gram-positive and -negative bacteria, including a fish pathogen, Aeromonas hydrophila, but no activity against fungi. The activity of the extracts was abolished by tryptic digestion, indicating that protein compounds were responsible for the antimicrobial activity. Furthermore, in a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging activity assay, only the visceral tissue extract showed activity. However, no extract had hemolytic activity against human red blood cells. Consequently, this study suggests the water-soluble extract of mottled anemone to be a promising source of proteinaceous antimicrobial compounds that can be utilized for development of novel antibiotics.
Bibliography:KISTI1.1003/JNL.JAKO201506362236408
ISSN:2234-1749
2234-1757