Epigallocatechin-3-gallate Inhibits Tax-dependent Activation of Nuclear Factor Kappa B and of Matrix Metalloproteinase 9 in Human T-cell Lymphotropic Virus-1 Positive Leukemia Cells

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 15; no. 3; pp. 1219 - 1225
• Main Authors: Harakeh, Steve, Diab-Assaf, Mona, Azar, Rania, Hassan, Hani Mutlak Abdulla, Tayeb, Safwan, Abou-El-Ardat, Khalil, Damanhouri, Ghazi Abdullah, Qadri, Ishtiaq, Abuzenadah, Adel, Chaudhary, Adeel, Kumosani, Taha, Niedzwiecki, Aleksandra, Rath, Mathias, Yacoub, Haitham, Azhar, Esam, Barbour, Elie
• Format: Journal Article
• Language: Korean
• Published: 2014
• Subjects: NF-kB pathway; Tax; human T-cell lymphotropic virus type I (HTLV-1); Acute T-cell leukemia
• ISSN: 1513-7368; 2476-762X

Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol molecule from green tea and is known to exhibit antioxidative as well as tumor suppressing activity. In order to examine EGCG tumor invasion and suppressing activity against adult T-cell leukemia (ATL), two HTLV-1 positive leukemia cells (HuT-102 and C91-PL) were treated with non-cytotoxic concentrations of EGCG for 2 and 4 days. Proliferation was significantly inhibited by 100 ${\mu}M$ at 4 days, with low cell lysis or cytotoxicity. HTLV-1 oncoprotein (Tax) expression in HuT-102 and C91-PL cells was inhibited by 25 ${\mu}M$ and 125 ${\mu}M$ respectively. The same concentrations of EGCG inhibited NF-kB nuclearization and stimulation of matrix metalloproteinase-9 (MMP-9) expression in both cell lines. These results indicate that EGCG can inhibit proliferation and reduce the invasive potential of HTLV-1-positive leukemia cells. It apparently exerted its effects by suppressing Tax expression, manifested by inhibiting the activation of NF-kB pathway and induction of MMP-9 transcription in HTLV-1 positive cells.