하고초(夏枯草)에서 추출한 Ursolic acid의 파골세포 분화 억제 효과

• Published in: 大韓韓方婦人科學會誌 Vol. 27; no. 2; pp. 59 - 70
• Main Authors: 허자경, 황덕상, 이진무, 이창훈, 장준복, 이경섭, Heo, Ja-Kyung, Hwang, Deok-Sang, Lee, Jin-Moo, Lee, Chang-Hoon, Jang, Jun-Bock, Lee, Kyung-Sub
• Format: Journal Article
• Language: Korean
• Published: 2014
• Subjects: C-Fos; Osteoclast; NFATc1; TRAP; Ursolic Acid; Prunella Vulgaris; OSCAR
• ISSN: 1229-4292; 2508-3619

Purpose: This study was conducted to evaluate the inhibitory effect of ursolic acid from Prunella vulgaris on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of ursolic acid from Prunella vulgaris in BMMs stimulated with M-CSF. TRAP staining, TRAP activity and Real-time PCR were performed to know the inhibitory effect on osteoclast differentiation. Actin ring formation were analysed to observe the effect of ursolic acid from Prunella vulgaris. Results: Ursolic acid from Prunella vulgaris has no cytotoxicity at the concentration of $1{\mu}g/ml$ or lower. Ursolic acid decreased the number of TRAP positive cells and the expression of NFATc1 gene, c-Fos gene, TRAP and OSCAR in BMMs stimulated with RANKL. Ursolic acid restrained the formation of actin ring. Ursolic acid inhibited NF-${\kappa}B$ activity by inducing degradation of p-$IkB{\alpha}$. Conclusions: Ursolic acid from Prunella vulgaris has the inhibitory effect of osteoclast differentiation and bone resorption. Futher studies are needed to treat osteoporosis by usolic acid from Prunella vulgaris.