Mechanisms of Hela Cell Apoptosis Induced by Abnormal Savda Munziq Total Phenolics Combined with Chemotherapeutic Agents

• Published in: Asian Pacific journal of cancer prevention : APJCP Vol. 15; no. 2; pp. 743 - 747
• Main Authors: Zhang, Yun-Xia, Abliz, Guzalnur, Ye, Wei-Jun, Mutalipu, Zuohelaguli, Li, Xiao-Wen, Wang, Hai-Qin, Buranjiang, Gulimire, Upur, Halmurat
• Format: Journal Article
• Language: Korean
• Published: 2014
• Subjects: survivin; Abnormal Savda Munziq total phenolics; Hela cells; Bcl-2 gene; apoptosis
• ISSN: 1513-7368; 2476-762X

Objective: To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined with cisplatin and docetaxel on the Hela cell growth. Methods: In vivo cultured Hela cells were treated with cisplatin, docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assess inhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test for survivin and Bcl-2 expression. Results: The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of $12.8{\pm}0.70%$, $18.9{\pm}3.79%$ and $15.8{\pm}3.8%$; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 and survivin (all P<0.01), especially when used in combination. Conclusion: ASMq total phenolics, combined with cisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression of Bcl-2 and survivin.