Purification and Characterization of A Novel Skatole-degrading Protease from Lactobacillus brevis 1.12

Skatole, a substance with an unpleasant flavor, is released from the cecum and colon of entire male pigs. In this study, a novel skatole-degrading protease from the fermentation broth of Lactobacillus brevis 1.12 was extracted and purified through $(NH_4)_2SO_4$ precipitation and chromatography with...

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Bibliographic Details
Published inFood science and biotechnology Vol. 22; no. 5; pp. 1367 - 1373
Main Authors Meng, Xiao, He, Zhi Fei, Li, Hong Jun
Format Journal Article
LanguageKorean
Published 2013
Subjects
12
skatole-degrading protease
purification
characterization
Lactobacillus brevis 1
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Summary:Skatole, a substance with an unpleasant flavor, is released from the cecum and colon of entire male pigs. In this study, a novel skatole-degrading protease from the fermentation broth of Lactobacillus brevis 1.12 was extracted and purified through $(NH_4)_2SO_4$ precipitation and chromatography with DEAE-Sephadex A-25 and Sephadex G-75. The purified protease was found to be homogeneous in terms of SDS-PAGE and had a molecular mass estimate of 57.5 kDa. This skatole-degrading protease had an optimal pH of 5.0 and optimal temperature of $35^{\circ}C$. The enzyme was only inhibited greatly at a 10 mmol 1/L concentration of $K^+$. The results suggest that the novel skatole-degrading protease was purified. The biochemical characteristics of this protease can provide useful information for its potential use in industrial applications.
Bibliography:KISTI1.1003/JNL.JAKO201319657434480
ISSN:1226-7708
2092-6456