{\alpha}$-Galactosidases Production by Debaryomyces hansenii UFV-1

Extracellular and intracellular ${\alpha}$-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with...

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Published inFood science and biotechnology Vol. 20; no. 3; pp. 601 - 606
Main Authors Viana, Pollyanna Amaral, Rezende, Sebastiao Tavares De, Passos, Flavia Maria Lopes, Machado, Solimar Goncalves, Maitan, Gabriela Picollo, Coelho, Vinicio Tadeu Da Silva, Guimaraes, Valeria Monteze
Format Journal Article
LanguageKorean
Published 2011
Subjects
Debaryomyces hansenii UFV-1
{\alpha}$-galactosidase
enzyme production
culture media
carbon source
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Summary:Extracellular and intracellular ${\alpha}$-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular ${\alpha}$-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon, such as described in Sacharomyces cereviseae.
Bibliography:KISTI1.1003/JNL.JAKO201120241367360
ISSN:1226-7708
2092-6456