대표적인 풍한열(風寒熱)에 의한 통증 치료 처방의 면역 활성화 비교 연구

• Published in: 동의생리병리학회지 Vol. 24; no. 4; pp. 616 - 623
• Main Authors: 정다영, 하혜경, 이호영, 이진아, 이준경, 황대선, 신현규, Jung, Da-Young, Ha, Hye-Kyung, Lee, Ho-Young, Lee, Jin-Ah, Lee, Jun-Kyoung, Huang, Dae-Sun, Shin, Hyeun-Kyoo
• Format: Journal Article
• Language: Korean
• Published: 2010
• Subjects: Bangpungtongsung-san (Fangfengtongsheng-san); Ohyaksungi-san (Wuyaoshunqi-san); Immune response; Ojeok-san (Wuji-san)
• ISSN: 1738-7698; 2283-2529

Three herbal formulas (Bangpungtongsung-san, Ohyaksungi-san, and Ojeok-san) for wind-cold and heat pain symptom were applied to investigate the immunological activities on antigen (Ag)-specific or Ag-non-specific immune responses in murine macrophage cell line (RAW 264.7) and ovalbumin (OVA)-immunized mice. This study was carried out in nitric oxide (NO) synthesis in RAW 264.7 cells and cellular proliferation in mouse splenocytes according to three herbal formulas. C57BL/6 mice were immunized intraperitonially with OVA/aluminium ($100\;{\mu}g/200\;{\mu}g$/mouse) on day 1, 8, and 15. Three herbal formulas were administrated to mice orally for 3 weeks from day 1. On day 22, OVA-, lipopolysaccharide (LPS)-, and concanavalin A (Con A)-stimulated splenocyte proliferation and antibodies (OVA-specific antibodies of the IgG, lgG1, and total IgM classes) in plasma were measured. Ohyaksungi-san increased NO synthesis in RAW 264.7 cells. Ojeok-san and Ohyaksungi-san significantly enhanced cellular proliferation by LPS and Con A in splenocytes from OVA-immunized mice (p<0.001). Three herbal formulas for wind-cold and heat pain symptom also significantly enhanced plasma OVA-specific IgG, IgG1, and total IgM levels compared with the OVA/Alum group. These results suggested that three herbal formulas for wind-cold and heat pain symptom could be used as stimulator of immune response.