LPS로 유도한 대식세포에서 MAP kinase의 억제에 의한 구보음(九寶飮)의 NO, TNF-$\alpha$, IL-6, IL-12 생성 억제 효과

• Published in: 동의생리병리학회지 Vol. 23; no. 1; pp. 104 - 112
• Main Authors: 이병순, 신조영, 이시형, Lee, Byung-Soon, Shin, Jo-Young, Lee, Si-Hyeong
• Format: Journal Article
• Language: Korean
• Published: 2009
• Subjects: GuBoEum(GBE); oriental medicine
• ISSN: 1738-7698; 2283-2529

The purpose of this study was to investigate the anti-inflammatory effects of extract from GuBoEum(GBE) on the peritoneal macrophage. To evaluate anti-inflammatory effects of GBE. I measured cytokines (interleukin-6; IL-6, interleukin-12; IL-12, tumor necrosis factor-$\alpha$; TNF-$\alpha$) and nitric oxide (NO) production in lipopolysacchride (LPS)-induced macrophages. Furthermore, I examined molecular mechanism using western blot and also LPS-induced endotoxin shock. Extract from GBE does not have any cytotoxic effect in the peritoneal macrophages. Extract from GBE reduced LPS-induced IL-6, TNF-$\alpha$, IL-12 and NO production in peritoneal macrophages. GBE inhibited the activation of extracelluar signal-regulated kinase (ERK), C-Jun $NH_2$-terminal kinase (JNK) but not of p38, degradation of $I{\kappa}B-{\alpha}$ in the LPS-stimulated peritoneal macrophages. GBE inhibited the production of TNF-$\alpha$, IL-6 and IL-12 in serum after LPS injection. These results suggest that GBE may inhibit the production of TNF-$\alpha$, IL-6, and IL-12 through inhibition of ERK and JNK activation, and that GBE may be beneficial oriental medicine for inflammatory diseases.