천초근 추출물이 HL-60 세포주의 세포자멸사 기전에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 22; no. 3; pp. 548 - 555
• Main Authors: 최호승, 박진모, 주성민, 김성훈, 김대근, 김원신, 전병훈, Choi, Ho-Seung, Park, Jin-Mo, Ju, Sung-Min, Kim, Sung-Hoon, Kim, Dae-Keun, Kim, Won-Sin, Jeon, Byung-Hun
• Format: Journal Article
• Language: Korean
• Published: 2008
• Subjects: Rubiae radix; p38; JNK; HL-60 cells; Apoptosis; Bax/Bcl-2 ratio; ERK
• ISSN: 1738-7698; 2283-2529

Rubiae radix belonging to the family Rubiaceae have been used in traditional medicine to blood stasis and hemostasis. In this study, we reported that methanol extract of Rubiae radix (RRME) induced apoptotic cell death through MAPKs activation in human promylocytic leukemia (HL-60) cells. The cytotoxic activity of activity of RRME in HL-60 cells was increased in a dose-dependent manner. RRME was cytotoxic to HL-60 cells, with IC50 of $8{\mu}g/mL$. Treatment of RRME to HL-60 cells showed apoptotic bodies, and the fragmentation of DNA, suggesting that these cells underwent apoptosis. Caspase-3 activity and PARP cleavage were time-dependently increased the expression of Bcl-2 and Bax. And ratio of Bax/Bcl-2 protein expression. Activation of p38 and JNK were increased 6 hr after RRME treatment in HL-60 cells, but activation of ERK was reduced 24 hr after treatment. Taken together, these results suggest that RRME induces apoptotic cell death through activation of p38 and JNK in HL-60 cells.