Temperature Controllable HPLC Column for Preparative Fractionation of Polymers

An HPLC column with a self-contained temperature control device was constructed for preparative temperature programmed interaction chromatography. Two Peltier plates were attached to a large bore column ($120{\times}22\;mm$ i.d.) and the column temperature was controlled by PID mode feed back contro...

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Bibliographic Details
Published inMacromolecular research Vol. 16; no. 6; pp. 544 - 548
Main Authors Im, Kyu-Hyun, Park, Hae-Woong, Kim, Young-Tak, Chang, Tai-Hyun
Format Journal Article
LanguageKorean
Published 2008
Subjects
temperature control
Peltier device
temperature gradient interaction chromatography
polymer fractionation
HPLC column
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Summary:An HPLC column with a self-contained temperature control device was constructed for preparative temperature programmed interaction chromatography. Two Peltier plates were attached to a large bore column ($120{\times}22\;mm$ i.d.) and the column temperature was controlled by PID mode feed back control. At a flow rate of 1.5 mL/min, the column temperature could be increased and decreased at a rate as high as $50^{\circ}C/min$ and $10^{\circ}C/min$, respectively, which is much faster than using a column jacket and bath/circulator. The rapid heating and cooling rates allows a high repetition rate of chromatographic fractionation. The performance of the temperature controllable column was demonstrated successfully by the fractionation of homo-polymer precursors from diblock copolymers.
Bibliography:KISTI1.1003/JNL.JAKO200820258462663
ISSN:1598-5032
2092-7673