Development and Characterization of a Specific Anti-Caveolin-1 Antibody for Caveolin-1 Functional Study in Human, Goat and Mouse

• Published in: Asian-australasian journal of animal sciences Vol. 20; no. 6; pp. 856 - 865
• Main Authors: Ke, Meng-Wei, Jiang, Yan-Nian, Li, Yi-Hung, Tseng, Ting-Yu, Kung, Ming-Shung, Huang, Chiun-Sheng, Cheng, Winston Teng-Kuei, Hsu, Jih-Tay, Ju, Yu-Ten
• Format: Journal Article
• Language: Korean
• Published: 2007
• Subjects: Caveolin-1; Peptide Antibody; Goat; Breast Cancer; Cancer Marker
• ISSN: 1011-2367; 1976-5517

Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-1 protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-1 and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.