B16-F10 Murine Melanoma 세포의 암전이 억제에 미치는 Diallyl Disulfide의 효과

• Published in: Journal of toxicology and public health Vol. 22; no. 4; pp. 349 - 356
• Main Authors: 강미경, 전혜승, 염영나, 황명실, 박미선, 김옥희, Kang, Mi-Kyung, Jun, Hye-Seung, Yum, Yung-Na, Hwang, Myung-Sil, Park, Mi-Sun, Kim, Ok-Hee
• Format: Journal Article
• Language: Korean
• Published: 2006
• Subjects: Diallyl disulfide; Invasion, Matrix metalloproteinase-2; B16F10 melamona cells; Experimental pulmonary metastasis assay
• ISSN: 1226-8399

Diallyl disulfide (DADS), an oil-soluble organosulfur compound in garlic has been reported to suppress tumor growth and to induce apoptosis in cancer. In the present study, we investigated the effects of DADS on pulmonary metastasis of B16-F10 murine melanoma cells. DADS (i.p. 40 mg/kg) significantly (p<0.05) reduced the number of pulmonary metastatic nodules (48%) in experimental pulmonary metastasis assay. We also found that DADS inhibited adhesion, invasion and migration of B16-F10 melanoma cells in a dose-dependent manner. To study the antimetastatic potential of DADS, we performed the effects of DADS on matrix metalloproteinase activity. DADS significantly inhibited the expression of matrix metalloproteinase-2 activity in B16-F10 cells by gelatin zymography. These results suggest that DADS prevent metastasis in part through suppression of migration of B16-F10 melanoma cells by Inhibiting matrix metalloproteirase-2 responsible for degradation of extracellar matrix.