Expressed sequence tags analysis of Blattella germanica

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editin...

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Bibliographic Details
Published inKorean journal of parasitology Vol. 43; no. 4; pp. 149 - 156
Main Authors CHUNG Hyang Suk, YU Tai Hyun, KIM Bong Jin, KIM Sun Mi, KIM Joo Yeong, YU Hak Sun, Jeong Hae Jin, OCK Mee Sun
Format Journal Article
LanguageKorean
Published 2005
Subjects
BLAST search
Blattella germanica
ESTs
cDNA library
novel genes
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Summary:Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About $42\%$ (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling path-ways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these rep-resent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.
Bibliography:KISTI1.1003/JNL.JAKO200509409830398
ISSN:0023-4001
1738-0006