Increased Production of Exoinulinase in Saccharomyces cerevisiae by Expressing the Kluyveromyces marxianus INU1 Gene Under the Control of the INU1 Promoter

Aspergillus niger inuE gene and Kluyveromyces marxianus INUI gene coding for exoinulinase were expressed in Saccharomyces cerevisiae under the control of K. marxianus INUI promoter. Recombinant S. cerevisiae expressing K. marxianus exoinulinase produced maximum 85 U/ml into culture medium, which was...

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Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 15; no. 2; pp. 447 - 450
Main Authors KIM HEE EUN, QIN RUI, CHAE KEON SANG
Format Journal Article
LanguageKorean
Published 2005
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Summary:Aspergillus niger inuE gene and Kluyveromyces marxianus INUI gene coding for exoinulinase were expressed in Saccharomyces cerevisiae under the control of K. marxianus INUI promoter. Recombinant S. cerevisiae expressing K. marxianus exoinulinase produced maximum 85 U/ml into culture medium, which was 9- to 14-fold higher than the activity produced by any other strain reported so far. In addition, K. marxianus INUI promoter produced 20- fold higher activity than S. cerevisiae glyceraldehydes phosphate dehydrogenase (GPD) promoter in S. cerevisiae.
Bibliography:KISTI1.1003/JNL.JAKO200502636834420
ISSN:1017-7825
1738-8872