사삼 메탄올 추출물의 멜라닌생성 억제효과

• Published in: 동의생리병리학회지 Vol. 18; no. 3; pp. 747 - 753
• Main Authors: 임난영, 권강주, 김윤석, 백순기, 임주락, 문연자, 우원홍, Lim Nan Young, Kwon Gang Joo, Kim Youn Seok, Baik Soon Ki, Lim Ju Rak, Mun Yeun Ja, Woo Won Hong
• Format: Journal Article
• Language: Korean
• Published: 2004
• Subjects: tyrosinase protein expression; melanin; melanogenesis; Adenophorae Radix
• ISSN: 1738-7698; 2283-2529

Effects of methanolic extract from Adenophorae Radix (AR) on melanogenesis were investigated in mouse melanoma B16F10 cells. The methanol extract of AR was partitioned into Hexane, Ethyl acetate (EA), Butanol, H₂O, and exogenously added to the culture medium for 72 hours at the concentration of 10, 50, 100 and 200 ㎍/㎖. Of the four partitions, Hexane and EA partion of AR reduced tyrosinase activity, which is the key enzyme for a melanogenesis, as well as melanin contents. But the EA partition was less toxic for B16F10 cells and has more efficient melanin-reducing effect than the former. In addition, the EA partition dramatically lightened the color of cell pellet and significantly decreased the level of tyrosinase protein expression. In these results, EA partition of AR reduced melanin synthesis of B16F10 mouse melanoma cells by down regulating the tyrosinase activity and tyrosinase protein expression. Therefore, it is anticipated that AR is a candidate for an efficient whitening agent which supresses melanogenesis.