Galactosylated Chitosan (GC)-graft-Poly(vinyl pyrrolidone) (PVP) as Hepatocyte-Targeting DNA Carrier: In Vitro Transfection

Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slight...

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Published inArchives of pharmacal research Vol. 27; no. 12; pp. 1284 - 1289
Main Authors Park, In-Kyu, Jiang, Hu-Lin, Cook, Seung-Eun, Cho, Myung-Haing, Kim, Su-Il, Jeong, Hwan-Jeong, Akaike, Toshihiro, Cho , Chong-Su
Format Journal Article
LanguageKorean
Published 2004
Subjects
Poly(vinyl pyrrolidone)
Gene delivery
Hepatocyte
Galactosylated chitosan
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Summary:Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of $Ca^{2+}$ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.
Bibliography:KISTI1.1003/JNL.JAKO200415875829714
ISSN:0253-6269
1976-3786