Anti-Inflammatory Mode of Isoflavone Glycoside Sophoricoside by Inhibition of Interleukin-6 and Cyclooxygenase-2 in Inflammatory Response

• Published in: Archives of pharmacal research Vol. 26; no. 4; pp. 306 - 311
• Main Authors: Kim, Byung-Hak, Chung, Eun-Yong, Ryu, Jae-Chun, Jung, Sang-Hun, Min, Kyung-Rak, Kim, Youngsoo
• Format: Journal Article
• Language: Korean
• Published: 2003
• Subjects: Cyclooxygenase; Sophora japonica; Sophoricoside; Anti-inflammation; Interleukin-6
• ISSN: 0253-6269; 1976-3786

Soy, high dietary intake for the oriental population, is a main source of isoflavonoids. Sophoricoside (SOP) an isoflavone glycoside was isolated from immature fruits of Sophora japonica (Leguminosae family) and its inhibitory effect on chemical mediators involved in inflammatory response was investigated in this study. SOP inhibited the interleukin (IL)-6 bioactivity with an $IC_{50}$ value of 6.1 $\mu$M whereas it had no effects on IL-1$\beta$ and TNF-a bioactivities. SOP was identified as a selective inhibitor of cyclooxygenase (COX)-2 activity with an $IC_{50}$ value of 4.4 $\mu$ M, but did not show inhibitory effect on the synthesis of COX-2. However, SOP had no effect on the production of reactive oxygen species including superoxide anions and nitric oxide. These results revealed that in vitro anti-inflammatory action of SOP is significantly different from that of genistein known as a phytoestrogen of soy products. This experimental study has documented an importance of dietary soy isoflavonoids as multifunctional agents beneficial to human health, and will help to clarify protective mechanisms of SOP against inflammatory conditions.