쥐에서 일과성 국소 뇌허혈 후 생긴 재관류 손상시 알부민치료의 효과

• Published in: 대한신경외과학회지 Vol. 30; no. 1; pp. 12 - 19
• Main Authors: 허필우, 조경석, 유도성, 김재건, 김달수, 강준기, Huh, Pil Woo, Cho, Kyoung Suck, Yoo, Do Sung, Kim, Jae Keon, Kim, Dal Soo, Kang, Joon Ki
• Format: Journal Article
• Language: Korean
• Published: 2001
• Subjects: Transient focal ischemia; Albumin; Thrombolysis; Reperfusion injury
• ISSN: 1225-8245; 1598-7876

Objective : Albumin is a very useful drug for the improving of cerebral blood volume and the oncotic effect in cerebral ischemia or cerebral vasospasm. The purpose of this study was to examine the morphological and neurological effect of albumin therapy on reperfusion injury following transient focal cerebral ischemia. Materials and Methods : 18 Male Sprague-Dawley rats weighing 270-320g were used. The ischemia model was produced by 2-hour period of transient middle cerebral artery occlusion with a poly-L-lysin coated intraluminal suture. The agent(20% human serum albumin[HSA]) or control solution(NaCl 0.9%) was administered intravenously at a dosage of 1% of body weight immediate after reperfusion following a 2-hour period occlusion. Neurological function was evaluated by the postural reflex and the forlimb placing test during occlusion(at 60 min) and daily for 3 days thereafter. The brain was perfusion-fixed, and infarct volumes and brain edema were measured. Results : The HSA significantly improved the neurological score in treated group. The rats of albumin treatment group showed significantly reduced total infarct volume(by 34%) and brain edema(by 81%) compared with salinetreated rats. Conclusion : HSA showed a substantial effect on the transient focal cerebral ischemia and reperfusion injury model. These results may indicate its usefulness in treating reperfusion injury patients after thrombolysis treatment for the thrombo-embolic major cerebral artery occlusions.