Expression of Chromium (VI) Reductase Gene of Heavy Metal Reducing Bacteria in Tobacco Plants

A Chromium (VI)[Cr(VI)] reductase gene from heavy metal reducing bacteria Pseudomonas aeruginosa HP014 was used to transform tobacco plant cells. A chimeric construct containing the Cr(VI) reductase gene was transfered to tobacco leaf disks using an Agrobacteriun tumefaciens binary vector system. Fr...

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Published inJournal of plant biotechnology Vol. 3; no. 1; pp. 13 - 17
Main Authors Jin, Tae-Eun, Kim, Il-Gi, Kim, Won-Sik, Suh, Suk-Chul, Kim, Byung-Dong, Rhim, Seong-Lyul
Format Journal Article
LanguageKorean
Published 2001
Subjects
transgenic tobacco plants
Pseudomonns aeruginosa HP104
chromium(VI) reductase gene
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Summary:A Chromium (VI)[Cr(VI)] reductase gene from heavy metal reducing bacteria Pseudomonas aeruginosa HP014 was used to transform tobacco plant cells. A chimeric construct containing the Cr(VI) reductase gene was transfered to tobacco leaf disks using an Agrobacteriun tumefaciens binary vector system. From the leaf disks, transformed plantlets were regenerated. Hybridization experiments demonstrated that the Cr(VI) reductase gene was inserted into and expressed in the regenerated plants. The Cr(VI) reduction activity showed that the transgenic plants may be a another possible tool to reduce the pollution of the toxic Cr(VI) in soil.
Bibliography:KISTI1.1003/JNL.JAKO200111921597038
ISSN:1229-2818
2384-1397