Quantitative Determination of Eleutheroside B and I from Acanthopanax Species by High Performance Liquid Chromatography

Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected In Korea. The stationary phase used was Zorbax 300 SB $C_{18}$ and a mobile phase program was used, which started at 6% acetonitrile...

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Bibliographic Details
Published inArchives of pharmacal research Vol. 24; no. 5; pp. 407 - 411
Main Authors Kang, Jong-Seong, Linh, Pham-Tuan, Cai, Xing-Fu, Kim, Hang-Sup, Lee, Jung-Joon, Kim, Young-Ho
Format Journal Article
LanguageKorean
Published 2001
Subjects
Gradient elution
Acanthopanax species
Eleutheroside E
Eleutheroside B
HPLC
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Summary:Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the various Acanthopanax species collected In Korea. The stationary phase used was Zorbax 300 SB $C_{18}$ and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and 1 in stems were higher than those In roots. Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.
Bibliography:KISTI1.1003/JNL.JAKO200111921097109
ISSN:0253-6269
1976-3786