Methed for the Passaging of Microcarrier Cultures to a Production Scale for Producing High Titre Disabled Infectious Single Cycle-Herpes Simplex virus Type-2

• Published in: Biotechnology and bioprocess engineering Vol. 5; no. 2; pp. 118 - 122
• Main Authors: Zecchini, Tracey-Ann, Wright, Paul-Andrew, Smith, Rodney-John
• Format: Journal Article
• Language: Korean
• Published: 2000
• Subjects: viral productivity; passaging regime; CR2 cells; culture passagime; DISC HSV-2, microcarrier; industrial manufacturing
• ISSN: 1226-8372; 1976-3816

A comlementary call line CR2 is curretly used to propagte the Disabled Infectious Single Cycle Herpes Simplex Virus Typee2 (DISC HSV-2) on a small Iaboratory scale upto 15 L. These cultures are initiated by passaging the cells from roller bottle cultures. Whilst this is suitable for the laboratory scale it is totally impractical for use in seeding an industrial manufacturing scaled version of the culture. It is paramount to have a robust system for passaging cells from a small microcarrierier culture system to a larger one by a serial subculturing regime. Here we report on the successes we have had in our laboratory in scaling up out production system for the DISC HSV-2 from small 1-L cultures to a 50-L vessel with the maintenance of the viral productivity. Ease of use, reproducibility and the need to minimise overall production time were factors which were taken into consideration whils developing our procedures. We were aware of the need to keep a production train simple and as short as possible as this was the amall scale study for an envisaged manufacturing process.