뇌신경교(腦神經膠) 성장세포(星狀細胞)로부터 종양괴사인자 알파의 생성(生成)에 있어서 총명탕(聰明湯)의 효과(效果)

• Published in: 동의신경정신과학회지 Vol. 10; no. 1; pp. 109 - 119
• Main Authors: 이종길, 강형원, 류영수, Lee Jong-Gil, Gang Hyeong-Won, Lyu Yeong-Su
• Format: Journal Article
• Language: Korean
• Published: 1999
• ISSN: 1226-6396; 2234-4942

We investigated whether an aqueous extract of Chong-Myung-Tang inhibits secretion of tumor necrosis $factor-{\alpha}$ $(TNF-{\alpha})$ from primary cultures of mouse astrocytes. Chong-Myung-Tang dosedependently inhibited the $TNF-{\alpha}$ secretion by astrocytes stimulated with substance P (SP) and lipopolysaccharide (LPS). Interleukin-1 (IL-1) has been shown to elevate $TNF-{\alpha}$ secretion from LPS-stimulated astrocytes while having no effect on astrocytes in the absence of LPS. We therefore investigated whether IL-1 mediated inhibition of $TNF-{\alpha}$ secretion from astrocytes by Chong-Myung-Tang. Treatment of Chong-Myung-Tang to astrocytes stimulated with both LPS and SP decreased IL-1 secretion. Moreover, incubation of astrocytes with IL-1 antibody abolished the synergistic cooperative effect of LPS and SP. These results suggest that Chong-Myung-Tang may inhibits $TNF-{\alpha}$ secretion by inhibiting IL-1 secretion and that Chong-Myung-Tang has a antiinflammatory activity in the central nervous system.