Effects of Brazilin on the Phospholipase $A_2$ Activity and Changes on Intracellular Free Calcium Concentration in Rat Platelets

Brazilin [7,11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10(6H)-tetrol] inhibited thrombin-, collagen- and ADP-induced aggregation of washed rat platelets. T hrombin- and collagen-induced ATP release were also inhibited by brazilin in a concentration-dependent manner. Brazilin inhibited the formation...

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Published inArchives of pharmacal research Vol. 21; no. 6; pp. 774 - 778
Main Authors Hwang, Gwi-Seo, Kim, Ji-Young, Chang, Tong-Shin, Jeon, Sun-Duck, So, Dhong-Su, Moon, Chang-Kiu
Format Journal Article
LanguageKorean
Published 1998
Subjects
Aggregation
Brazilin
Caesalpinia sappan
Platelt
Phospholipase $A_2
Intracellular free calcium
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Summary:Brazilin [7,11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10(6H)-tetrol] inhibited thrombin-, collagen- and ADP-induced aggregation of washed rat platelets. T hrombin- and collagen-induced ATP release were also inhibited by brazilin in a concentration-dependent manner. Brazilin inhibited the formation of platelet thromboxane $A_2$ caused by thrombin, whereas it had no effect on the prostaglandin $D_2$ formation. Brazilin inhibited $^3H$-arachidonic acid liberation from membrane phospholipids of thrombin-stimulated platelets. Brazilin inhibited the rise of intracellular free calcium caused by thrombin. These results indicate that the inhibition of phospholipase ($PLA_2$) activity and [$[Ca^{2+}]_1$ elevation might be at least a part of antiplatelet mechanism of brazilin.
Bibliography:KISTI1.1003/JNL.JAKO199815875837774
ISSN:0253-6269
1976-3786