CO-CULTURE OF BOVINE EMBRYOS WITH CUMULUS CELLS

Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of cul...

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Bibliographic Details
Published inAsian-australasian journal of animal sciences Vol. 2; no. 4; pp. 595 - 598
Main Authors Goto, K, Koba, M, Takuma, Y, Nakanishi, Y, Ogawa, K
Format Journal Article
LanguageKorean
Published 1989
Subjects
Embryo
Bovine
Co-Culture
In-Vitro Fertilization
Cumulus Cells
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Summary:Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of culture, respectively, while embryos/ova cultured in medium alone failed to develop beyond 8-cell (0-13.3%), morula (0-1.5%) and blastocyst stages (0%). The results of this study demonstrated the beneficial effect of cumulus cells on the development of bovine embryos.
Bibliography:KISTI1.1003/JNL.JAKO198923443162860
ISSN:1011-2367
1976-5517