상추 균핵병 억제 및 생장촉진을 위한 길항 세균의 효능

This study was conducted to biochemically characterize and screen the efficacy of antagonistic bacterial isolates in suppressing sclerotinia rot in lettuce crops. A total of 123 bacterial isolates were isolated from soil. In vitro antagonistic screening showed that 13 bacterial isolates were effecti...

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Published in강원 농업생명환경연구 Vol. 34; no. 3; pp. 413 - 424
Main Authors 김현승, Hyun Seung Kim, 마헤시아드히카리, Adhikari Mahesh, 박효빈, Hyo Bin Park, 김기영, Gi Young Kim, 이인규, In Gyu Lee, 변은정, Eun Jeong Byeon, 우지민, Ji Min Woo, 이윤수, Youn Su Lee
Format Journal Article
LanguageKorean
Published 강원대학교 농업생명과학연구원 31.12.2022
Subjects
Bacillus megaterium
Biochemical characterization
Inhibition
Sclerotinia rot
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Summary:This study was conducted to biochemically characterize and screen the efficacy of antagonistic bacterial isolates in suppressing sclerotinia rot in lettuce crops. A total of 123 bacterial isolates were isolated from soil. In vitro antagonistic screening showed that 13 bacterial isolates were effective against Sclerotinia sclerotiorum and S. minor. In particular, two strains of Bacillus megaterium (HCS-1B and WJR-6B) showed the highest effectiveness against the tested pathogens, with a 75-80% inhibition rate. Additionally, the 13 bacterial isolates were biochemically characterized. Ammonia production, phosphate solubilization, protease production, starch hydrolysis and siderophore production, and HCN production were observed in 13, 3, 11, 9, and 2 bacterial isolates, respectively. Based on the results of the in vitro antagonistic test and biochemical characterization, the two best bacterial isolates (HCS-1B and WJR-6B) were selected for sclerotinia rot suppression and lettuce growth promotion under greenhouse conditions. The lowest incidence rate (0%) of sclerotinia rot was recorded in WJR-6B-treated lettuce pre-inoculated with S. sclerotiorum and in S. sclerotiorum-inoculated lettuce pretreated with HCS-1B. Combination treatment of HCS-1B and WJR-6B increased the leaf area of lettuce by approximately 3-fold (218.4 cm 2 ) compared to the control (75 cm 2 ). In this study, HCS-1B and WJR-6B were identified as potential antagonistic bacterial isolates that inhibit sclerotinia rot. Field testing of these potential bacterial isolates would be a good direction for future studies.
Bibliography:Institute of Agricultural Science Kangwon National University
ISSN:2233-8322