Bombyx mori b-tubulin Promoter for High-level Expression of Heterologous Genes

We previously isolated 9 clones that show stronger signal compared to Bombyx mori cytoplasmic actin gene (BmA3) by using a dot blot hybridization. In this study, we focused on one clone among these clones which has high amino acid similarity with b-tubulin gene of B. mori. This clone was ubiquitousl...

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Bibliographic Details
Published inInternational Journal of Industrial Entomology Vol. 39; no. 1; pp. 22 - 28
Main Authors Kwanho Park, Tae-won Goo
Format Journal Article
LanguageKorean
Published 한국잠사학회 30.09.2019
Subjects
BmA3
Bombyx mori
Luciferase assay
Promoter
β-tubulin
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Summary:We previously isolated 9 clones that show stronger signal compared to Bombyx mori cytoplasmic actin gene (BmA3) by using a dot blot hybridization. In this study, we focused on one clone among these clones which has high amino acid similarity with b-tubulin gene of B. mori. This clone was ubiquitously expressed in all tissues and developmental stage of B. mori. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-750/-1) in the 5’-flanking region of b-tubulin gene, which has about 47 fold more intensive promoter activity than BmA3 promoter. Moreover, the b-tubulin promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that b-tubulin promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter. © 2019 The Korean Society of Sericultural Sciences Int. J. Indust. Entomol. 39(1), 22-28 (2019)
Bibliography:Korean Society of Sericultural Science
ISSN:1598-3579