Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of β-Galactosidase-Containing Escherichia coli

We investigated whether β-galactosidase (β-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molec...

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Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 24; no. 9; pp. 1254 - 1259
Main Authors Hyang Yeol Lee, Kyung Hwan Jung
Format Journal Article
LanguageKorean
Published 한국미생물생명공학회 30.09.2014
Subjects
2-phenoxyethanol
2-phenoxyethanol galactoside
Escherichia coli
transgalactosylation
β-galactosidase
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Summary:We investigated whether β-galactosidase (β-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molecule was covalently bonded with PE during thin-layer chromatography analysis of the β-gal hydrolysate of PE-Gal. The yield for PE-Gal synthesis was about 37.5% (weight basis), which was about 7-8 times greater than that of a previous report. In addition, the concentration of β-gal (0.96 U/ml) used in this PE-Gal synthesis was about 20 times less than that in a previous report.
Bibliography:The Korean Society for Applied Microbiology
ISSN:1017-7825