하고초(夏枯草)에서 추출한 Ursolic acid의파골세포 분화 억제 효과

• Published in: 대한한방부인과학회지 Vol. 27; no. 2; pp. 59 - 70
• Main Authors: 허자경, Ja Kyung Heo, 황덕상, Deok Sang Hwang, 이진무, Jin Moo Lee, 이창훈, Chang Hoon Lee, 장준복, Jun Bock Jang, 이경섭, Kyung Sub Lee
• Format: Journal Article
• Language: Korean
• Published: 대한한방부인과학회 25.05.2014
• Subjects: C-Fos; NFATc1; OSCAR; Osteoclast; Prunella Vulgaris; TRAP; Ursolic Acid
• ISSN: 1229-4292

This study was conducted to evaluate the inhibitory effect of ursolicacid from Prunella vulgaris on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of ursolic acidfrom Prunella vulgaris in BMMs stimulated with M-CSF. TRAP staining, TRAPactivity and Real-time PCR were performed to know the inhibitory effect onosteoclast differentiation. Actin ring formation were analysed to observe the effectof ursolic acid from Prunella vulgaris. Results: Ursolic acid from Prunella vulgaris has no cytotoxicity at the concentrationof 1 μg/ml or lower. Ursolic acid decreased the number of TRAP positive cells and the expression of NFATc1 gene, c-Fos gene, TRAP and OSCAR in BMMs stimulatedwith RANKL. Ursolic acid restrained the formation of actin ring. Ursolic acidinhibited NF-κB activity by inducing degradation of p-IkBa.Conclusions: Ursolic acid from Prunella vulgaris has the inhibitory effect ofosteoclast differentiation and bone resorption. Futher studies are needed to treatosteoporosis by usolic acid from Prunella vulgaris.