Experimental Research Article : Antioxidant effect of muscle relaxants (vecuronium, rocuronium) on the rabbit abdominal aortic endothelial damage induced by reactive oxygen species

• Published in: Korean journal of anesthesiology Vol. 65; no. 6; pp. 552 - 558
• Main Authors: Ji Seon Jeong, Jung Kook Suh, Eun Sun Cho, Dong Won Kim, Mi Ae Jeong
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회(구 대한마취과학회) 30.12.2013
• Subjects: Antioxidant effect; Reactive oxygen species; Rocuronium; Vecuronium
• ISSN: 2005-6419; 2005-7563

Background: Muscle relaxants induce vascular smooth muscle relaxation by inducing synthesis of the prostaglandins that influence vasomotor tone. However, the effects of muscle relaxants on endothelial cells and tissues following injury by reactive oxygen species (ROS) are unclear. We tested the effects of the muscle relaxants vecuronium and rocuronium on impaired acetylcholine (ACh)-induced relaxation following induction of ROS in rabbit aorta in vitro. Methods: Isolated rabbit abdominal aortic ring segments were pretreated with vecuronium or rocuronium at 10-4, 3 × 10-4, 10-3 or 3 × 10-3 M, with or without inhibitors of Cu/Zn superoxide dismutase (diethyldithiocarbamate; DETCA, 0.8 mM) or catalase (3-amino-1,2,4-triazole; 3AT, 50 mM). All groups of aortic rings were then exposed to ROS generated by electrolysis in the organ bath medium (Krebs-Henseleit solution). The effects of vecuronium and rocuronium on ROS-induced impairment of relaxation induced by ACh (10-6 M) were assessed. Results: Aortic rings treated with vecuronium or rocuronium at 10-4, 3 × 10-4, 10-3 or 3 × 10-3 M preserved the capacity for ACh-induced endothelial relaxation following ROS exposure in a dose-dependent manner. Pretreatment with DETCA partially inhibited the protective effects of vecuronium and rocuronium on ACh-induced relaxation (P < 0.001), but pretreatment with 3AT had no effect. Conclusions: Muscle relaxants protected the endothelium in isolated rabbit abdominal aorta from free-radical injury in a dose-dependent manner. These results suggest that vecuronium and rocuronium may act as superoxide anion scavengers. (Korean J Anesthesiol 2013; 65: 552-558)