Epigallocatechin-3-gallate Inhibits LPS-Induced NF-κB and MAPK Signaling Pathways in Bone Marrow-Derived Macrophages

• Published in: Gut and liver Vol. 6; no. 2; pp. 188 - 196
• Main Authors: So Young Joo, Young A Song, Young Lan Park, Eun Myung, Cho Yun Chung, Kang Jin Park, Sung Bum Cho, Wan Sik Lee, Hyun Soo Kim, Jong Sun Rew, Nack Sung Kim, Young Eun Joo
• Format: Journal Article
• Language: Korean
• Published: 대한소화기학회 30.04.2012
• Subjects: Epigallocatechin-3-gallate; Macrophage; Mitogen-activated protein kinase; Nuclear factor-κB
• ISSN: 1976-2283

Background/Aims: Epigallocatechin-3-gallate (EGCG), the primary catechin in green tea, has anti-inflammatory and anti-oxidative properties. The aim of the current study was to characterize the impact of EGCG on lipopolysaccharide (LPS)-induced innate signaling in bone marrow-derived macrophages (BMMs) isolated from ICR mice. Methods: The effect of EGCG on LPS-induced pro-infl ammatory gene expression and nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling was examined using reverse transcription-polymerase chain reaction, Western blotting, immunofl uorescence, and the electrophoretic mobility shift assay. Results: EGCG inhibited accumulation of LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA in BMMs. EGCG blocked LPS-induced IκBα degradation and RelA nuclear translocation. EGCG blocked the DNA-binding activity of NF-κB. LPS-induced phosphorylation of ERK1/2, JNK, and p38 was inhibited by EGCG. U0126 (an inhibitor of MEK- 1/2) suppressed the LPS-induced IL-12p40, IL-6, MCP-1, ICAM-1, and VCAM-1 mRNA accumulation in BMMs. Conclusions: These results indicate that EGCG may prevent LPSinduced pro-infl ammatory gene expression through blocking NF-κB and MAPK signaling pathways in BMMs. (Gut Liver 2012;6:188-196)