A549 기도상피세포에서 IL-8 전사와 분비에 미치는 Dexamethasone과 Troglitazone의 병합투여효과

• Published in: Chʻŏnsik mit alrerugi Vol. 28; no. 4; pp. 292 - 297
• Main Authors: 김남희, Nam Hee Kim, 이호연, Ho Youn Lee, 서지현, Ji Hyun Suh, 한성환, Sung Hwahn Hahn, 김도형, Doh Hyung Kim, 김윤섭, Youn Seop Kim, 박재석, Jae Seuk Park, 지영구, Young Koo Jee
• Format: Journal Article
• Language: Korean
• Published: 대한천식알레르기학회 30.12.2008
• Subjects: Peroxisome proliferator activated receptor γ; Steroid; Troglitazone
• ISSN: 1226-8739

Background: Glucocorticoid is a widely used anti-inflammatory agent in a variety of diseases. Recently, peroxisome proliferator activated receptor γ (PPARγ) ligand has been shown to exert a potential anti-inflammatory activity, mainly through their ability to down- regulate pro-inflammatory gene expressions. Objective This study was designed to evaluate the effects of co-treatment with dexamethasone and troglitazone (a ligand of PPARγ) on IL-8 transcription and secretion. Method: Steroid and troglitazone-induced transactivation was measured by using pGRE luciferase reporter gene in the A549 cell line. Steroid-induced transrepression was measured by using stable A549 IgG-NFκB luciferase cell line, which contained the minimal IL-8 promoter region. Result: Co-treatment with dexamethasone and troglitazone showed no additional increase of transactivation activity compared to dexamethasone single treatment. Suppressions of the transcriptional activity in the IL-8 promotor and IL-8 secretion showed no difference in the co-treatment with dexamethasone and troglitazone compared to those in dexamethasone single treatment. Conclusion: Our results suggest that co-treatment with PPARγ ligand and steroid may not have an additive anti-inflammatory activity at least in the airway epithelial cell. (Korean J Asthma Allergy Clin Immunol 2008;28:292-297)